Figure 1

Effect of 100 μM NMMA, 1 mM SNAP (SNP), 1 mM nitroprusside (NTP), 5 mM tempol, 10 μM indomethacin (Indo), or 1 μM BAPTA on AVP (1 nM) – stimulated cAMP accumulation in acutely isolated IMCD from CD ET-1 KO and floxed (control) mice. All data are expressed as percent of cAMP accumulation with AVP alone in control IMCD. Control IMCD AVP-stimulated cAMP accumulation was 141 ± 9 fmol cAMP/μg total cell protein (n = 18). *p < 0.05 compared to floxed controls treated with the same condition, **p < 0.01 vs. control + no inhibitor. N = 6–18 each data point.