Effects of uremic solutes on reactive oxygen species in vitro model systems as a possibility of support the renal function management

Table 5 Relations between the IC ₅₀ of the solutes uric acid, phenol and p -cresol determined in the crocin bleaching assay, with single solutes or binary mixtures

Mixtures of uremic solutes	Volume (μL) of solute mixture needed for IC ₅₀	Concentrations (μmol/L) of each solute in mixture at IC ₅₀			Total concentration of uremic solutes observed in the mixture (μmol/L)	IC ₅₀ (μmol/L) of single solutes			Projected total concentration (μmol/L) of the binary mixtures of uremic solutes from the IC ₅₀ of a single solutes*
		Uric acid	Phenol	p -Cresol		Uric acid	Phenol	p -Cresol
Uric acid + Phenol	150.3	5.18	846.04	---	851.22	6.90	1125.81	---	566.35
Uric acid + p -cresol	126.5	4.36	---	735.16	739.52	6.90	---	1162.31	584.60
Phenol *+ p* -cresol	152.5	---	858.43	886.26	1744.69	---	1125.81	1162.31	1144.06

*The projected total concentration at the IC₅₀ of the binary mixtures, for example, uric acid + phenol is (6.90 + 1125.81)/2 = 566.35.

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