Figure 1

Angptl3 affected the PAN-induced podocyte detachment. (a) The detachment was determined in the podocytes at different doses (50,100,200,250 and 500 ng/ml) of rm-Angptl3 with or without PAN (50 μg/ml, 10 hrs) pretreatment for 24 hrs, it shown that rm-Angptl3 without PAN could not change the attached cells in normal podocytes significantly, while, in podocytes pretreated with PAN, rm-Angptl3 reduced the attached cells markedly, and the more the doses (100-500 ng/ml), the less the attached cells. (b) The rates of attached cells podocytes treated with rm-Angptl3 for different times after PAN pretreatment; the cells decreased from 9 h to 48 h, and the rate of 24 h was the lowest. (c) ELISA results showing weaker secretion of Angptl3 in the medium of Angptl3 siRNA group (lowered by 69.50%); (d) Western blot confirmed that Angptl3 was knocked down by siRNA successfully (reduced by 86.89 ± 3.58%). (e) There was no statistical difference between cell number in the Wt group and control siRNA group with or without PAN pretreatment. Cell number in the control siRNA group and Angptl3 siRNA group were almost the same, while, after PAN pretreatment, cell number in the Angptl3 siRNA group is more than in the control siRNA group. (Wt: wide type; Control siRNA: podocytes transfected with control siRNA; Angptl3 siRNA: podocytes transfected with Angptl3 siRNA. *P <0.05, **P <0.01, ***P <0.0001) Angptl3, angiopoietin-like3; ELISA, enzyme-linked immunosorbent assay; PAN, puromycin aminonucleoside; siRNA, small interfering RNA.