Angptl3 affected apoptosis in PAN-induced podocyte injury model. (a) Flow cytometry analysis of the rate of apoptosis in the cultured podocytes with different treatments. No significant differences in the number of apoptotic cells were observed among control, rm-Angptl3, control siRNA and Angptl3 siRNA groups. After PAN pretreatment, the rate of apoptosis was significantly increased in the rm-Angptl3 group compared to the control group (34.80±7.9 vs. 1.89±0.92 3%, P<0.0001; n=6). And in comparison to the control siRNA group, apoptosis rate in the podocytes treated with Angptl3 siRNA was a little lower (9.29±1.27vs. 17.42±2.61%, P<0.05; n=6). (b, c) This finding was confirmed using TUNEL assay for apoptosis in cultured podocytes. In PAN-induced podocyte injury model, TUNEL positive cells in rm-Angptl3-treating group are significantly more than that without rm-Angptl3 treating (56±9 vs. 8±10 %, P<0.0001; n=6), and TUNEL positive cells in the Angptl3 siRNA group are significantly less than that in control siRNA group (32±4 vs. 54±9 %, P<0.05; n=6) (magnification ×400). (Control: podocytes treated without rm-ANGPTL3; Rm-ANGPTL3: podocytes treated with rm-ANGPTL3. *P<0.05, ***P<0.0001). Angptl3, angiopoietin-like3; PAN, puromycin aminonucleoside; siRNA, small interfering RNA; TUNEL. deoxynucleotidyl transferase-mediated dUTP nick-end labeling.