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Figure 3 | BMC Nephrology

Figure 3

From: A vital role for Angptl3 in the PAN-induced podocyte loss by affecting detachment and apoptosis in vitro

Figure 3

Angptl3 influenced the F-actin rearrangement in podocytes. (a) Phalloidin fluorescence labelling of F-actin showed podocyte stress fibers. Confocal microscopy showed no difference in the F-actin staining pattern between control group and Angptl3 siRNA group. However, F-actin rearrangement in Angptl3 group, characterized by the formation of lamellipodia (arrowhead) and increase in cell spikes (arrow). In PAN induced podocyte injury model,cells became retracted and F-actin seemed partial disrupted,moreover, F-actin almost completely disrupted in Angptl3 protein group. However, disruption did not occur in Angptl3 knockdown group. (b) The percentage of stress fibers also showed that Angptl3 protein could significantly aggravate the effect of PAN on decreaseing podocyte stress fibers while Angptl3 siRNA could prevent PAN from decreasing podocyte stress fibers. (c) Quantitative analysis showed the MFI of F-actin in different groups under confocal microscopy. After PAN treatment, MFI value in the Angptl3 siRNA group is higher than in the control siRNA group. (Scale bar, 5 μm *P <0.05, **P <0.01. n=100). Angptl3, angiopoietin-like3; MFI, mean fluorescence intensity; PAN, puromycin-aminonucleoside; siRNA, small interfering RNA.

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