Figure 3

Effect of β2-M treatment on EMT in HK-2 cells. (A) HK-2 cells were stimulated with 25 μM β2-M for 72 h, Cell lysates were immunoblotted with antibodies against E-cadherin, α-SMA, fibronectin, vimentin, and β-actin. (B) Graphical representation of E-cadherin, α-SMA, vimentin, and fibronectin protein abundances as indicated. The relative E-cadherin, α-SMA, vimentin, and fibronectin levels were calculated after normalization to β-actin. The data are presented as the mean ± SE of three experiments. **P < 0.05 versus controls.