Figure 5

The β2-M/HFE complex modulated iron levels and induced HIF-1α expression. (A) Iron levels in HK-2 cells and HFE knockdown cells were measured by inductively coupled plasma mass spectroscopy. (B) Western blot analysis of HIF-1α in HK-2 cells and HFE knockdown cells. (C) Graphical representation of relative HIF-1α protein abundances after normalization with β-actin as indicated. The data are presented as the mean ± SE of three experiments. **P < 0.05 versus controls.