Figure 6

Effect of iron on EMT in HK-2 cells. (A) HK-2 cells were stimulated with DES for 48 h, and photographs were taken using a Nikon TMS microscope at 400x magnification. (B) HK-2 cells were cultured in the presence or absence of DES for 48 h. Cell lysates were immunoblotted with antibodies against HIF-1α, E-cadherin, vimentin, and α-SMA. (C) Graphical representation of relative HIF-1α, E-cadherin, vimentin, and α-SMA protein abundances after normalization with β-actin as indicated. The data are presented as the mean ± SE of three experiments. **P < 0.05 versus controls.