Fig. 4

NF-κB-dependent luciferase reporter activity and TLR4 expression in normal and ADPKD cells. a Endogenous NF-κB activity in HK-2, WT9-7 and WT9-12 cells, 24 h post-transfection. **p < 0.01 vs. HK-2, ##p < 0.01 vs. 9–7 cells. Data are expressed as mean + SD from 3 experiments. b NF-κB activity was stimulated by 4 h of incubation with LPS (10 μg/mL) or TNF-α (20 ng/mL). NF-κB activity is expressed as the fold-change in luciferase/GFP over untreated control. *p < 0.05 vs. vehicle control for the corresponding cell line, **p < 0.01 vs. vehicle control for the corresponding cell line. Data are expressed as mean + SD from 3 experiments. c Expression of TLR4 in HK-2, WT9-7 and WT9-12 cells, determined by western blotting in whole cell lysates. d PDTC decreased TNF-α-induced NF-κB activity in HK-2 but not in WT9-7 or WT9-12 cells. Data are expressed as mean + SD from 2 experiments. **p < 0.01 vs. vehicle control for the corresponding cell line; ##p < 0.01