Fig. 2

Proliferative and migratory phenotype of CoRL in UUO. (a–c) To detect proliferation in CoRL, double-staining was performed for Red fluorescent protein (red) to identify CoRL, and BrdU (green) to examine cell proliferation. Nuclei are labeled with DAPI (blue). Medulla: (a) CoRL did not co-express BrdU in sham kidneys. ( b) At day 7 post UUO, a subset of CoRL co-expressed BrdU (yellow color; arrow shows example). The inset demonstrates this at higher magnification. As expected, other peritubular cells stained for BrdU (open arrowheads). (c) Some dual CoRL⁺/BrdU⁺ cells displayed elongated shape with long cellular processes suggesting cell motility when viewed at high power (inset). (d) To better define the subset of labeled CoRL that appeared to be migrating based on their shape (identified by RFP staining, arrowheads), double staining was performed for the endothelial cell marker CD31 (green, arrows). (d’) On occasion, CoRL were detected within vessels in the medulla