Fig. 3

Interstitial CoRL co-express pericytes markers. To label pericytes, antibody staining against PDGFRß (green) and NG2 (red) was performed, CoRL were identified by RFP staining, nuclei are labeled with DAPI. (a – c) Cortex: (a) Representative images show that in sham-operated mice, CoRL co-express pericyte markers in JG (arrowhead). Insets show high power images of juxtaglomerular CoRL. In UUO, there was an accumulation of interstitial pericytes (orange color). Interstitial CoRL were found in peritubular areas co-expressing pericyte markers at (b) 7 day post UUO, and (c) 14 days post UUO. Insets show high power images of CoRL. (d–f) Medulla: (d) Sham medulla shows CoRL co-expressing pericyte markers in vasa recta (arrowheads). Insets show high power images. Pericyte marker staining increases in UUO (orange color). Interstitial CoRL were co-labeled with pericyte markers at (e) day 7 post UUO, (f) day 14 post UUO. Insets show high power images of interstitial CoRL