Fig. 1From: Membranoproliferative glomerulonephritis recurrence after kidney transplantation: using the new classificationHistological changes of MPGN in kidney transplant biopsies. Typical light microscopic (LM), electron microscopy (EM) and immunofluorescence (IF) finding in cases previously classified as MPGN. Panel on left demonstrates a case reclassified as ICGN with C3 abnormalities, including (a) the classic MPGN pattern glomerulonephritis on LM (hematoxylin and eosin ×400), (b) large subendothelial electron dense deposits on EM (×1250 K) and granular mesangial and capillary wall staining for both (c) IgG and (d) C3 on IF. The panel on the right shows a case reclassified as a C3 glomerulopathy, with (e) a similar MPGN pattern on light microscopy (hematoxylin and eosin ×400), (f) smaller subendothelial deposits on EM (×7100) and granular mesangial and capillary wall staining for (g) C3, but no significant staining for (h) IgG. EM images stained with lead citrate/ uranyl acetate. Immunofluorescence stains are FITC-conjugated goat anti-human IgG (MP Biomedical) and FITC-conjugated goat anti-human C3 (Kent) all at × 400Back to article page