Fig. 5
From: Changes in renal WT-1 expression preceding hypertension development
a Histological sections of neonatal SHR kidney cortices during hypertension development. In newborn SHRs (0 W), WT-1, VDR and Hsp70 immunostaining (expression) was relatively high, and it decreased in the cytoplasm of tubule epithelial cells and in glomeruli, while AT1 staining was observed in the same epithelial duct segments. After 4 weeks of hypertension evolution, decreased WT-1/VDR/Hsp70 immunostaining levels were seen in cortex tubule cells, and there was higher AT1 staining in tubule cells (4 W vs. 0 W). Finally, after 8 weeks of hypertension development, a greater decrease in WT-1/VDR/Hsp70 immunostaining levels were seen in cortex tubule cells, compared to what was seen in newborn SHRs (8 W vs. 0 W). Also, a very high level of AT1 staining in the tubule cells and glomeruli of the 8-week-old SHR was observed (8 W vs. 0 W). Magnification: 400X. b Histological sections of neonatal WKY kidney cortices. In newborn WKYs (0 W to 8 W), WT-1, VDR and Hsp70 immunostaining (expression) was relatively high, while an AT1 low staining was observed in the same epithelial duct segments. Magnification: 400X