Fig. 6
From: Changes in renal WT-1 expression preceding hypertension development
a Immunofluorescence confocal microscopy study in SHRs. Immunofluorescence/cytochemical localization of WT-1, VDR, Hsp70 and AT1 in kidney cortices from SHR rats. Renal cortex tissues were labeled with antibodies against WT-1, VDR, Hsp70 and AT1 followed by anti-rabbit fluorescein isothiocyanate-conjugated, and anti-mouse Rhodamine Red and Green X-conjugated secondary antibodies. Nuclei were stained with Hoechst 33342 (blue). Images represent five different experiments. In tubule epithelial cells of the newborn SHR (0 W), greater WT-1 (red), VDR (green) and Hsp70 (red) staining were identified. Similarly, basal expression of AT1 (green) appears in the tubule epithelial cells. Surprisingly, at week 4 of hypertension development, immunoreactive WT-1, VDR and Hsp70 decreased in the cytosol and the membrane of epithelial tubule cells. In addition, at week 8, these parameters were very low. Contrarily, at week 4 of hypertension development, immunoreactive AT1 in the cytosol and the membrane of epithelial tubule cells increased, and at week 8, it was very high. Magnification: 600X. b Immunofluorescence confocal microscopy study in WKYs. Immunofluorescence/cytochemical localization of WT-1, VDR, Hsp70 and AT1 in kidney cortices from WKY rats. Renal cortex tissues were labeled with antibodies against WT-1, VDR, Hsp70 and AT1 followed by anti-rabbit fluorescein isothiocyanate-conjugated, and anti-mouse Rhodamine Red and Green X-conjugated secondary antibodies. Nuclei were stained with Hoechst 33342 (blue). Images represent five different experiments. In tubule epithelial cells of the newborn WKY (0 W to 8 W), high WT-1 (red), VDR (green) and Hsp70 (red) staining were identified. In addition, a low expression of AT1 (green) appears in the tubule epithelial cells. Interesting, at week 8, immunoreactive WT-1, VDR and Hsp70 not decreased in the cytosol and the membrane of epithelial tubule cells. Also, at week 8, immunoreactive AT1 not increased in the cytosol and the membrane of epithelial tubule cells. Magnification: 600X