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Fig. 3 | BMC Nephrology

Fig. 3

From: Mesangial cells from patients with IgA nephropathy have increased susceptibility to galactose-deficient IgA1

Fig. 3

Gene expression and release of IL-6. IL-6 expression is known to increase in mesangial cells when stimulated with gd-IgA and has been suggested as a prognostic marker for IgAN but its role is still debated. Mesangial cells (MCs) from patients with IgA nephropathy (IgAN) and controls were stimulated with IgA1 purified from blood from healthy controls (cIgA) or patients with IgAN (gd-IgA) or medium only for 6 h and the gene expression was investigated. Stimulation with IgA on both controls MCs and IgAN MCs increased the gene expression of IL-6 in a similar pattern (a). The release of IL-6 into the medium after 24 h of stimulation was most pronounced for the gd-IgA treatment for both groups of cells, but the IgAN MCs released significantly more IL-6 than the control MCs stimulated with gd-IgA (b). Grey bars represent control MCs, black bars represent IgAN MCs * P < 0.05, ** P < 0.01, ***P < 0.001, error bars represent SEM

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