Fig 6

Dendrin mediated RhoA/mDia/YAP deficiency-induced podocyte apoptosis. a Dendrin protein expression was knockdown to about 35 % in Den-siRNA treated podocytes. b-i Con-siRNA, Den&RhoA-siRNA, Den&mDia-siRNA, Den&YAP-siRNA were short for control siRNA, Dendrin-siRNA plus RhoA-siRNA, Dendrin-siRNA plus mDia-siRNA, Dendrin-siRNA plus YAP-siRNA respectively. Podocytes were stained with Annexin V/PI for flow cytometry analysis. Cell apoptosis rate was obviously increased in RhoA, mDia or YAP knockdown podocytes. Knocking down dendrin expression with siRNA significantly abolished RhoA, mDia or YAP deficiency-induced podocyte apoptosis. All data were from at least three independent experiments. * P < 0.05 vs con-siRNA group. # P < 0.05