Fig. 4

G-CSF treatment decreased numbers of splenic immune cells. a Absolute number of total splenocytes was lower in the G-CSF group (P < 0.01). b Numbers of B cell (CD19⁺), T cells (CD3⁺), and T cell subsets (CD4⁺_, CD8⁺) were decreased by G-CSF treatment. c-e Number of CD11b⁺ myeloid cells (c) was not significantly decreased by G-CSF, whereas numbers of CD11c⁺ dendritic cells (d) and NK cells (NK1.1⁺CD3^-, e) were significantly lower in the G-CSF group. f-g Numbers of both follicular helper T cells (CD4⁺CXCR5⁺PD-1⁺, f) and plasma cells (CD19^lowCD138^hi, g) were lower in the G-CSF group. Flowcytometric analysis was used for quantifying spleen cell types. The data are expressed as the mean ± standard error of the mean, with n = 4 − 5 mice per group. White and black bars indicate the G-CSF group and the PBS group, respectively. *P < 0.05, **P < 0.01 for comparisons between the G-CSF and the PBS groups using t-test. G-CSF: granulocyte colony-stimulating factor; PBS: phosphate-buffered saline