Skip to main content
Fig. 7 | BMC Nephrology

Fig. 7

From: Alteration of histone H3K4 methylation in glomerular podocytes associated with proteinuria in patients with membranous nephropathy

Fig. 7

Effect of MLL3 shRNA on the expression of histone H3K4 me3 and cathepsin L in the LPS model. Expression of H3K4 me3 was determined by Western blot (a and b) and an ELISA using material isolated by acid extraction (c). The upregulated expression of H3K4 me3 (a–c) and cathepsin L (d and e) was significantly lower in MLL3 shRNA compared with control shRNA in the LPS-treated mice. GFP protein detected by immunohistochemistry using an anti-GFP antibody was clearly detected on the podocytes of kidneys transfected with GFP shRNA (f). *P < 0.05 vs. Veh. LPS, lipopolysaccharide treated mice; Veh, vehicle treated mice; H3K4 me3, histone H3K4 trimethylation; total H3, total histone H3; N = 10. Data are expressed as the mean ± SE

Back to article page