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Fig. 5 | BMC Nephrology

Fig. 5

From: Endothelial Colony Forming Cells (ECFCs) in murine AKI – implications for future cell-based therapies

Fig. 5

Endothelial p62 expression. Firstly, ischemia induced endothelial p62 in all experimental groups. Nevertheless, levels were always higher in the 45 than in the 35 min groups. Secondly, cell therapy failed to modulate endothelial p62 at any given timepoint with the only exception of 35 min at week 1 (a). bn display representative images from controls and several experimental groups. The white rectangle in (i) surrounds areas of CD31/p62 colocalization. Images (kn) show the results from confocal co-localization analysis with the respective monochromatic and one merged image(s). k and n: p62 shows a punctuated staining pattern within CD31+ cells but also within adjacent vascular wall cells (magnifications × 40 in b - i; ×160 in j; ×630 in k - n; green in all microscopic images: p62; red: CD31; yellow: CD31/p62 overlap; blue: nuclei; Data as mean ± SEM, ✻: p < 0.05)

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