Fig. 5
From: Renal effects of metallothionein induction by zinc in vitro and in vivo
a-c Median mRNA expression of MTT: The effect of different hypoxic environments (III, IV) compared to non-hypoxic conditions (I, II) with different Zn-concentrations. Zn: Zinc. Zn 5: 5 μg/ml (17.4 μM); Zn 10: 10 μg/ml (34.8 μM); Zn 15: 15 μg/ml (52.2 μM). Cell culture environments: I: 4.5 h normal conditions in cell culture cabinet (5% CO2, 18% O2, 37 °C); II: 24 h normal conditions in cell culture cabinet (5% CO2, 18% O2, 37 °C); III: 4.5 h hypoxia cell culture cabinet (5% CO2, 3% O2, 37 °C); IV: 24 h hypoxia cell culture cabinet (5% CO2, 3% O2, 37 °C); V: 4.5 h hypoxia cell culture cabinet (5% CO2, 3% O2, 37 °C) and 19.5 h normal conditions in cell culture cabinet (5% CO2, 18% O2, 37 °C)