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Table 3 Median mRNA expression of MTT dependent on different Zinc concentrations under different hypoxic (III, IV) and non-hypoxic (I, II) environments. Cell culture environments: I: 4.5 h normal conditions in cell culture cabinet (5% CO2, 18% O2, 37 °C); II: 24 h normal conditions in cell culture cabinet (5% CO2, 18% O2, 37 °C); III: 4.5 h hypoxia cell culture cabinet (5% CO2, 3% O2, 37 °C); IV: 24 h hypoxia cell culture cabinet (5% CO2, 3% O2, 37 °C); V: 4.5 h hypoxia cell culture cabinet (5% CO2, 3% O2, 37 °C) and 19.5 h normal conditions in cell culture cabinet (5% CO2, 18% O2, 37 °C)

From: Renal effects of metallothionein induction by zinc in vitro and in vivo

 

Median MTT expression (95% CI)

Zinc concentration

Control

5 μg/ml (17.4 μM)

10 μg/ml (34.8 μM)

15 μg/ml (52.2 μM)

p-value

Environment

Normal I

1.90 (1.49–2.43)

5.22 (4.09–6.66)

11.56 (9.06–14.76)

34.94 (26.85–45.47)

0.089

Hypoxia III

1.53 (1.20–1.95)

4.18 (3.28–5.34)

9.27 (7.26–11.83)

28.00 (21.52–36.44)

Normal II

0.59 (0.46–0.75)

1.62 (1.27–2.05)

3.58 (2.82–4.55)

10.81 (8.44–13.85)

0.23

Hypoxia IV

0.50 (0.39–0.63)

1.37 (1.08–1.74)

3.03 (2.38–3.85)

9.15 (7.15–11.72)

Normal II

0.59 (0.46–0.75)

1.62 (1.27–2.05)

3.58 (2.82–4.55)

10.81 (8.44–13.85)

0.25

Hypoxia V

0.51 (0.40–0.65)

1.39 (1.09–1.78)

3.08 (2.42–3.93)

9.32 (7.16–12.12)