Fig. 5

Number of bolus injections used to help support blood pressure. Rats of the I/R control group and all citrate groups were subjected to clamping of both renal vascular pedicles for 40 min followed by three hours of reperfusion as diagrammed in Fig. 1. Animals of the citrate groups received sodium citrate at indicated concentrations 60 min before and 45 min after ischemia. Calcium levels were kept stable by corresponding infusions of calcium gluconate. Normoxic and I/R control groups received only 0.9% NaCl solution. Infusion volume was 5 ml/kg/h in all animals. Additional bolus injections of 0.5 ml 0.9% NaCl were administered repetitively up to a maximum volume of 5 ml/kg/h at a mean arterial blood pressure below 70 mmHg for more than 5 min. Box plots with whiskers indicating minimum and maximum, six rats per group. a Bolus injections during ischemia. # p < 0.05 and ## p < 0.01 vs. normoxic control group. ** p < 0.01 vs. I/R control group. b Bolus injections during reperfusion. # p < 0.05 and ### p < 0.001 vs. normoxic control group. * p < 0.05 and ** p < 0.01 vs. I/R control group. 1 Bolus injection = 0.5 ml 0.9% NaCl