Fig. 4
From: Sustained hyperosmolarity increses TGF-ß1 and Egr-1 expression in the rat renal medulla
Effect of chronic hyperosmolarity on the expression of profibrotic genes and transcription factors in IMCD cells. a The progressive increase of medium osmolarity induced overexpression of the Egr-1 mRNA. Mild type III collagen upregulation was induced only by 900 mOsm, dramatic (6-fold) type IV collagen overexpression was observed at medium osmolarity of 600 mOsm. b Egr-1 and TGF-ß mRNA expressions showed the strongest correlation. Both type III and type IV collagen expressions correlated well to the expression of the profibrotic Egr-1. c Components of the transcription factor AP-1, cFos and cJun, were strongly overexpressed only at high osmolar concentration of 900 mOsm. Data are presented as mean ± SEM, n = 4/group. * p < 0.05, ** p < 0.01 (Kruskal-Wallis test with Dunn’s post-hoc test)