Table 1 Primer sequences, product size and annealing temperatures used in RT-PCR
Gene | Product size (pb) | Temperature (°C) | ||
|---|---|---|---|---|
β-actin | Right | GTTGTCGACGACGAGCG | 93 | 60 |
Left | GCACAGAGCCTCGCCTT | 50 | ||
E-cadherin | Right | GACCGGTGCAATCTTCAAA | 93 | 59 |
Left | TTGACGCCGAGAGCTACAC | 60 | ||
α-SMA | Right | GCGTGATTTCCAGCACATAA | 101 | 59 |
Left | ATACTTGACCGGGGTCATCC | 60 |