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Fig. 6 | BMC Nephrology

Fig. 6

From: Injury induced expression of caveolar proteins in human kidney tubules - role of megakaryoblastic leukemia 1

Fig. 6

Control of caveolar proteins by megakaryoblastic leukemia 1 (MKL1). a. Western blots showing protein levels of caveolin-1/CAV1 and PTRF/CAVIN1 at different cell densities and at 3 and 5 days post-confluence in the absence (−) or presence (+) of 10 μM CCG-1423. Quantification of Western blot data from CCG-1423 treatment at 5 days post-confluence are shown in (b) (n = 4). c. Results of qPCR showing caveolin-1/CAV1 and PTRF/CAVIN1 mRNA levels after viral transduction with different multiplicities of infection (MOI) of Ad-CMV-MKL1. Panels d and e show qPCR for caveolin-2/CAV2 and SDPR/CAVIN2 at 100 MOI of Ad-CMV-null or Ad-CMV-MKL1. *(p < 0.05), **(p < 0.01), ***(p < 0.001). f. Western blots for the MKL1 coactivator FLNA and repressor SCAI, comparing levels in normal kidney cortical tissue lysates and cultured kidney epithelial cells obtained from the same kidneys 5 days post confluency. As caveolin-1/CAV1 and PTRF/CAVIN1 expression becomes evident during culture, a parallel de novo expression of FLNA is seen. Conversely, the pronounced cortical levels of SCAI are diminished during culture

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