Fig. 1
Procedure for producing H2-dissolved peritoneal dialysis solution by the bathing method. a Time-course of changes in dissolved H2 levels in electrolyzed water (*) and the solution in the PD bag (#). b Time-course of changes in dissolved H2 levels in the solution in the PD bag when placed in air. Water was electrolyzed by Nafion (Synthetic polymer membrane; DuPont, Wilmington, DE) to generate highly dissolved H2 water, with the concentration of H2 exceeding 1.5 ppm. Then, the PD bag was bathed in the water to allow H2 to shift into the PD solution by diffusion (a). Dissolved hydrogen is lost rapidly after loading once it is exposed to room air (b). Thus, the H2-dissolved PD solution was subjected to experiments within 1 h of 12-h bathing, to ensure a high level of H2 in the PD solution (>400 ppm)