Assessment of the association between increasing membrane pore size and endotoxin permeability using a novel experimental dialysis simulation set-up

Table 2 Overview of the experimental steps of the in vitro dialysis

Experimental Step	Solution Blood side	Circulation blood side	Time (min)	Q_B (ml/min)	Q_D (ml/min)	Q_F (ml/min)	Infusion Contaminant
Priming Blood circuit	NaCl	Discard	5	100 ml/min	0	0	none
Priming Dialysate circuit	None	–	2	0	500	0	none
Exchange fluid in Blood circuit	Plasma	Discard	~2^a	200	0	0	0
Coating	Plasma	Recirculate	40	200	0	0	0
Rinsing	PBS 1×	Discard	18	200	0	30	0
Exchange fluid in Blood circuit	PVP	Discard	~2^a	200	0	0	0
Dialysis	PVP	Recirculate	60	400	500	0	10 ml/min

^atime depends on volume of circuit + membrane. For Polyflux: 1′56″; Revaclear: 1′53″; Theranova: 1′52″; Theralite: 2′07″

ISSN: 1471-2369