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Fig. 1 | BMC Nephrology

Fig. 1

From: MORG1+/− mice are protected from histological renal damage and inflammation in a murine model of endotoxemia

Fig. 1

a-e: Analysis of renal damage. a Detection of renal injury by PAS- staining 24 h post LPS administration. LPS treatment induced cortical tubular dilatation in MORG1+/+ mice. Representative images are shown. The vasodilation and brush border damages are shown with asterisk on the images. Original magnification 400×. Bars correspond to 20 μm. N = 6 per group. b Semi-quantitative cortical tubular injury scores. The score ranges from 0 to 5 depending on level of tubular damage. N = 6, MORG1+/+/LPS vs. MORG1+/+ saline treated mice, p < 0.001; MORG1+/+/LPS vs. MORG1+/−/LPS, p = 0.017. *p < 0.05, ***p < 0.001. c Detection of the tubular damage by immunohistochemical detection of the Kidney injury molecule 1 (KIM1) protein expression in renal sections 24 h post endotoxemia or saline treatment. Representative images are shown. Original magnification 400×. Bars 20 μm. N = 4. d Number of KIM1 positive tubuli per kidney section are graphically presented. N = 4 mice per group. MORG1+/+/LPS versus MORG1+/−/LPS, p = 0.029, *p < 0.05. MORG1+/+/LPS vs. MORG1+/+/saline, ***p < 0.001. MORG1+/−/LPS vs. MORG1+/−/saline, ***p < 0.001. e Real-time PCR analyses of the Kim1 mRNA expression in endotoxemic and saline treated wild-type and MORG1+/− mice. LPS induced a robust increase in the Kim1 mRNA expression in both genotypes. Nevertheless, a down-regulation of MORG1 was associated with a significantly lower expression of Kim1 compared with the wild-type endotoxemic mice. N = 4 MORG1+/+/ NaCl; N = 8 MORG1+/+/LPS; N = 7 MORG1+/−/NaCl; N = 5 MORG1+/−/LPS. MORG1+/+/LPS vs. MORG1+/−/LPS p = 0.0097, **p < 0.01

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