Fig. 2
a-f: Analysis of renal functional parameters 24 h following endotoxemia induction or saline solution application. a Determination of the plasma concentration of Neutrophil gelatinase associated lipocaline (NGAL). N = 6 per group, ***p < 0.001. b Measurement of the NGAL urine concentration in 24 h collected urine. At least 6 animals per group were subjected to the analysis. MORG1+/− LPS vs. MORG1+/− NaCl treated mice, p = 0.002; MORG1+/+ LPS vs. MORG1+/+ NaCl treated mice, p < 0.001. **p < 0.01, ***p < 0.001. c Determination of the renal NGAL mRNA expression via real-time PCR analyses. The expression is presented as a ratio relative to the saline treated MORG1+/+ mice. N = 4 per group, MORG1+/+ LPS vs. MORG1+/− LPS, p = 0.021; MORG1+/− LPS vs. MORG1+/− NaCl treated mice p = 0.049; MORG1+/+ LPS vs. MORG1+/+ NaCl treated mice, p < 0.001. *p < 0.05, ***p < 0.001. d Detection of renal injury by determination of urinary ACR (uACR). LPS treatment significantly increased the uACR in MORG1+/+ and MORG1+/− mice compared with the corresponding controls, p = 0.008 and p < 0.001 respectively; the levels of uACR are significantly lower in endotoxemic MORG1+/− mice relatively to the equally treated MORG1+/+ mice, p = 0.03. N = 6 per group. *p < 0.05, **p < 0.01, ***p < 0.001. e Detection of plasma creatinine (Cre) levels 24 h following endotoxemia or saline application. N = 7 per group for MORG1+/+ saline and MORG1+/− saline, N = 9 mice per group for MORG1+/+ LPS and MORG1+/− LPS. LPS induced increase in plasma Cre in both genotypes compared with the control saline treated groups. MORG1+/+ LPS vs. MORG1+/+ saline treated mice, p = 0.012, MORG1+/− LPS vs. MORG1+/−saline *p = 0.0156, *p < 0.05. f Detection of plasma blood urea nitrogen (BUN) concentrations 24 h following endotoxemia or saline application. N = 7 per group for MORG1+/+ saline and MORG1+/− saline, N = 9 mice per group for MORG1+/+ LPS and MORG1+/− LPS. Endotoxemia elevated the plasma BUN concentrations in both genotypes compared with the control saline treated groups. MORG1+/+ LPS vs. MORG1+/+ saline treated mice, p = 0.001, MORG1+/− LPS vs. MORG1+/−saline p = 0.004, **p < 0.01, **p < 0.001