Fig. 6

a-c: Tnfα mRNA expression in renal tissue. a Influence of LPS on renal expression of Tnfα mRNA in MORG1^+/+ and MORG1^+/− mice was analysed by real-time PCR 24 h post LPS application. N = 6, MORG1^+/+ LPS vs. MORG1^+/− LPS treated mice, p = 0.003; (MORG1^+/+ LPS vs. MORG1^+/+ saline treated mice, p < 0.001), MORG1^+/− LPS vs. MORG1^+/− saline treated mice, p < 0.001. **p < 0.01, ***p < 0.001. b Detection of the protein expression of TNF-α by Western blotting using whole kidney protein lysates. The protein expression of TNF-α in LPS treated MORG1^+/− mice was significantly lower than in endotoxemia- exposed MORG1^+/+ mice. Representative images are shown. Four independent experiments were conducted. N = 4. c The expression of the proteins was quantified with ImageJ. TNF-α expression levels were normalized to vinculin loading and are graphically presented relative to NaCl treated MORG1^+/+ mice. N = 4 per group, MORG1^+/+ LPS vs. MORG1^+/+ saline treated mice, p = 0.008; MORG1^+/+ LPS vs. MORG1^+/− LPS treated mice, p = 0.004. **p < 0.01