Fig. 7
a-f: Analyses of NF-κB signalling in endotoxemia mice. a Assessment of IκB-α phosphorylation in renal tissue 24 h after induction of endotoxemia or saline. Phospho-IκB-α levels in total kidney protein lysate were detected by Western blot analyses. The expression of IκB-α is also shown. Equal loading was detected by analyses of the vinculin protein expression. Representative images are shown. Four independent experiments were performed. b The expression of the proteins was analysed by ImageJ software. pIκB-α expression levels were normalised to vinculin loading and are graphically presented. N = 4 per group, MORG1+/+ LPS vs. MORG1+/+ saline treated mice, p = 0.024; MORG1+/+ LPS vs. MORG1+/− LPS treated mice, p = 0.048. *p < 0.05. c Detection of IKKα,β phosphorylation in renal tissue. Phospho-IKK-α,β levels in total kidney protein lysate was detected by Western blot. Equal loading was monitored by detection of the vinculin protein expression. Representative images are shown. N = 4. d The expression of the proteins was analysed by ImageJ software. pIKK-α,β expression levels were normalized to IKK-α expression and are graphically presented relative to NaCl treated MORG1+/+ mice. N = 4 per group, MORG1+/+ LPS vs. MORG1+/− LPS treated mice, p < 0.05, MORG1+/+ LPS vs. MORG1+/+ saline treated mice, p < 0.01. *p < 0.05, **p < 0.01. e Detection of NF-κB (p65) protein in the nuclear fraction of kidney lysates of NaCl and LPS injected MORG1+/+ and MORG1+/− mice by Western blot. Equal loading was monitored by assessment of the PCNA nuclear protein expression. Representative images are shown. Three independent experiments were performed. N = 3. f The expression of the proteins was analysed by ImageJ software. NF-κB nuclear levels were normalised to PCNA expression and are graphically presented relative to NaCl treated MORG1+/+ mice. N = 3 per group, MORG1+/+ LPS vs. MORG1+/− LPS treated mice p = 0.002; MORG1+/+ LPS vs. MORG1+/+ saline treated mice, p < 0.001. **p < 0.01, ***p < 0.001