Fig. 8

a-d: Reduced MORG1 expression ameliorates the peritubular infiltration of inflammatory T-cells in renal tissue and reduced renal caspae-3 cleavage in endotoxemic mice 24 h post LPS application. a Immunohistological detection of CD3⁺ cells. Representative images are shown. The CD3⁺ cells are shown with asterisk on the images. The nuclei were counter-stained with DAPI. The Original magnification 400×. Bars correspond to 20 μm. N = 6 per group. b Evaluation of CD3⁺ expression by scoring method. N = 6 per group, MORG1^+/+ LPS vs. MORG1^+/+ saline treated mice, p = 0.003; MORG1^+/+ LPS vs. MORG1^+/− LPS treated mice, p < 0.001. **p < 0.01, ***p < 0.001. c Analysis of caspase-3 activation by Western blot in kidney lysates cytosolic fraction of NaCl and LPS injected MORG1^+/+ and MORG1^+/− animals. Activation of caspase-3 was revealed by the presence of the 17 and 12 kDa protein bands, corresponding to the cleaved (activated) caspase-3. Equal loading was controlled by vinculin expression. Representative images from three independent experiments are shown. N = 3. d The proteins levels of cleaved caspase-3 were measured by ImageJ software, normalized to vinculin expression and are graphically presented relative to NaCl treated MORG1^+/+ mice. N = 3 per group, MORG1^+/+ LPS vs. MORG1^+/+ saline treated mice, p = 0.002; MORG1^+/+ LPS vs. MORG1^+/− LPS treated mice, p = 0.004. **p < 0.01, ***p < 0.001