Fig. 2
IP3 and MCU agonists induced podocyte mitochondrial Ca2+ overload and apoptosis in vitro. Ctl, control; IP3: D-myo-inositol 1, 4, 5-triphosphate tripotassium salt; Spm: Spermine; a, compared with the Ctl group; b, compared with the IP3 group; **, P < 0.01; *, P < 0.05. a The cells in Q3 which were annexin high and PI low were counted as apoptotic cells. Compared with the Ctl podocytes, IP3 (n = 9) and Spm (n = 3) all induced significant increase of the podocyte apoptosis rate. b Compared with the Ctl podocytes, IP3 and Spm all induced significant increase of mitochondrial Ca2+ in mouse podocytes (n = 12). The increase of mitochondrial Ca2+ induced by IP3 was partially prevented by EDTA