Fig. 5From: IP3R-Grp75-VDAC1-MCU calcium regulation axis antagonists protect podocytes from apoptosis and decrease proteinuria in an Adriamycin nephropathy rat modelKnocking down Grp75 protected podocytes from ADR- and Ang II-induced apoptosis in vitro. Ctl, control; Ang II, angiotensin II; Grp75, glucose-regulated protein 75; siCtl, non-targeted negative control siRNA; siGrp75, siRNA to knock down Grp75; a, compared with the Ctl group; b, compared with the ADR or Ang II group; *, P < 0.05; **, P < 0.01. a Knocking down Grp75 decreased Grp75 compared with non-targeted negative control siRNA group; b) The cells in Q3 which were annexin high and PI low were counted as apoptotic cells. Compared with ADR or Ang II -treated podocytes, knock down of Grp75 decreased the podocyte apoptosis rate significantly. c Compared with ADR or Ang II -treated podocytes, knock down of Grp75 decreased active caspase-3 significantly. d Compared with ADR or Ang II -treated podocytes, knock down of Grp75 decreased mitochondrial Ca2+ levels significantlyBack to article page