Table 2 Nested PCR primers for analysis ATP6V1B1 exon 8 skipping
name | Forward primer (5′–3′) | Reverse primer (5′–3′) | Product (bp) |
|---|---|---|---|
Exon8-1P | ATCCTACGAACTCCGGTGTC | TATCGTCGTTGGGCATGGTG | 730 bp |
Exon8-2P | GAGATGATTCAGACGGGCAT | CACCTCCTCTCTAGCAGCAG | 450 bp |
Exon8-3P | ATGAGATTGCCGCTCAGAT | GCATAGGAACTCATGTCCGT | 313 bp |