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Table 2 Oligonucleotide primer sets for real-time PCR

From: Gut derived-endotoxin contributes to inflammation in severe ischemic acute kidney injury

Gene Forward Primer Reverse Primer
β-actin 5’-AGATTACTGCCCTGGCTCCTAG-3’ 5’-CATCGTACTCCTGCTTGCTGA-3’
TNF-α 5’-TGCCTCAGCCTCTTCTCATT-3’ 5’-GGGCTTGTCACTCGAGTTTT-3’
IL-6 5’-AGTTGCCTTCTTGGGACTGA-3’ 5’-ACAGTGCATCATCGCTGTTC-3’
MCP-1 5’-GATGCAGTTAATGCCCCACT-3’ 5’-TTCCTTATTGGGGTCAGCAC-3’