Table 2 Oligonucleotide primer sets for real-time PCR
From: Gut derived-endotoxin contributes to inflammation in severe ischemic acute kidney injury
Gene | Forward Primer | Reverse Primer |
|---|---|---|
β-actin | 5’-AGATTACTGCCCTGGCTCCTAG-3’ | 5’-CATCGTACTCCTGCTTGCTGA-3’ |
TNF-α | 5’-TGCCTCAGCCTCTTCTCATT-3’ | 5’-GGGCTTGTCACTCGAGTTTT-3’ |
IL-6 | 5’-AGTTGCCTTCTTGGGACTGA-3’ | 5’-ACAGTGCATCATCGCTGTTC-3’ |
MCP-1 | 5’-GATGCAGTTAATGCCCCACT-3’ | 5’-TTCCTTATTGGGGTCAGCAC-3’ |