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Fig. 3 | BMC Nephrology

Fig. 3

From: Meprin β metalloproteases associated with differential metabolite profiles in the plasma and urine of mice with type 1 diabetes and diabetic nephropathy

Fig. 3

Scores plots for multivariate statistical analyses of metabolites in plasma. Blood samples were collected at 4-week post STZ injection by tail nick and 8-week by cardiac puncture and processed to obtain plasma. Panel a: Unsupervised analysis principal components analysis (PCA) demonstrating overall structure and variability within the large LC-MS dataset for all, 4 week only and 8 week only plasma samples in either positive or negative electrospray ionization modes. Panel b: Side-by-side scores plots for the unsupervised PCA and supervised OPLS-DA multivariate analyses for each genotype in positive mode. Panel c: Side-by-side scores plots for the unsupervised PCA and supervised OPLS-DA multivariate analyses for each genotype in negative mode. These plots visualize (along with Tables 1, 2 and 3 inputs and fit statistics to aid interpretation) the STZ treatment effect in the data subset PCA and the effectiveness of OPLS-DA to uncover metabolite variables (loadings) capable of discriminating the two treatment groups

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