Fig. 2

Inhibition of S1PR2 reverses HG-induced endothelial cell dysfunction. HEGECs were treated as described in Fig. 1. a The cell permeability was assessed by measuring the FITC-BSA that crossed the monolayer, and quantified by a fluorescence plate reader. b HRGECs were stained with both Annexin V and propidium iodide (PI), then determined using flow cytometric analysis. c The degree of migration of different groups was quantified by microscope. d The expression levels of RhoA, ROCK1, and Drp1 were evaluated by Western blot analysis. The band densities of these proteins were quantified with GAPDH as a loading control. Results are expressed as mean ± SD of three independent experiments. *P < 0.05 versus the HG group; ^#P < 0.05 versus the NG group