Fig. 3

Inhibition of ROCK1 reverses HG-induced mitochondrial fission and dysfunction. HEGECs were pretreated with the ROCK1 inhibitor Y27632 for 30 min and then incubated with normal glucose (NG) or high glucose (HG) for 72 h. Drp1 protein expression (a), mitochondrial morphology (b), intracellular ATP production (c), ROS generation (d), and Ca2+ concentration (e) were assessed as described in Fig. 1. Results are expressed as mean ± SD of three independent experiments. *P < 0.05 versus the HG group; ^#P < 0.05 versus the NG group. EM, electron microscopy; LM, local magnification