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Table 4 Effect of hypertonic saline and furosemide on excretion of proteins from epithelial sodium channels and aquaporin-2 channels in a randomized, cross-over study of 24 healthy subjects

From: Effect of 3% saline and furosemide on biomarkers of kidney injury and renal tubular function and GFR in healthy subjects – a randomized controlled trial

Period

Baseline

Hypertonic saline infusion

Post hypertonic saline infusion

 
 

0–90 min

90–150 min

150–180 min

180–210 min

210–240 min

240–300 min

P (GLM within)

U-AQP2 (ng/minute)

 Placebo

0.81 (0.66;0.93)

0.85 (0.71;1.05)

1.00 (0.81;1.31)*

1.01 (0.87;1.38)*

1.07 (0.77;1.26)*

0.98 (0.80;1.09)*

< 0.001

 Furosemide

0.77 (0.66;0.92)

1.42 (1.18;1.61)*

1.12 (0.90;1.41)*

1.14 (0.88;1.46)*

0.86 (0.72;1.10)*

0.79 (0.72;0.93)

P (GLM between)

0.553

 

U-AQP2 /creatinine (ng/mmol)

 Placebo

72 (66;84)

84 (74;87)

86 (83;104)*

102 (85;111)*

90 (77;99)*

92 (82;98)*

< 0.001

 Furosemide

76 (63;83)

140 (104;150)*

100 (91;129)*

121 (84;132)*

97 (81;107)*

88 (64;104)*

P (GLM between)

0.186

 

U-ENaCγ (ng/minute)

 Placebo

0.87 (0.71;1.27)

0.73 (0.60;1.19)

0.90 (0.76;1.27)

0.81 (0.70;1.10)

0.75 (0.64;1.13)

0.68 (0.60;1.03)*

0.399

 Furosemide

0.92 (0.83;1.26)

0.87 (0.75;1.03)

1.06 (0.63;1.31)

0.83 (0.64;1.12)

0.81 (0.64;1.04)*

0.73 (0.63;0.93)*

P (GLM between)

0.806

 

U-ENaCγ /creatinine (ng/mmol)

 Placebo

80 (72;97)

80 (63;90)

84 (72;98)

79 (63;87)

70 (63;89)

69 (62;81)*

0.884

 Furosemide

91 (82;99)

75 (66;131)

81 (70;116)

88 (72;16)

83 (69;107)*

72 (60;97)*

P (GLM between)

0.487

 

UAER (μg/min)

 Placebo

1 (0;5)

3 (3;4)

4 (4;6)

4 (3;6)

4 (3;4)

3 (0;4)

0.129

 Furosemide

1 (0;5)

0 (0;9)

0 (0;10)

4 (1;7)

4 (2;6)

3 (2;5)

P (GLM between)

0.167

 
  1. u-AQP2/minute Aquaporin-2 excretion rate, U-AQP2/creatinine creatinine adjusted u-AQP2 excretion, u-ENaCγ/minute excretion of the γ-fraction of the epithelial sodium channel and U-ENaCγ /creatinine creatinine adjusted u-ENACγ, UAER urinary albumin excretion rate. Urine was collected every 30 min in the 90 min baseline period, once after 60 min of hypertonic infusion, and every 30 min 90 min after hypertonic saline infusion and once 150 min after cessation of hypertonic saline infusion. Data from three baseline periods are pooled and shown as one period. Data are shown as medians with 25 and 75 percentiles in brackets. P-value represents probability of difference in response to hypertonic saline (response from baseline to hypertonic saline) between treatments Statistics are performed with a general linear model (GLM), or Wilcoxon signed rank test. Data were logarithmic transformed before GLM was performed. Difference from baseline: * = p < 0.05