Fig. 2

Renal structural injury induced by ischemia/reperfusion was prevented by pirfenidone administration. a) Urinary output, b) urinary microalbumin/creatinine ratio (MA/Cr), c) urinary Hsp72 levels by Western blotting and d) densitometric analysis of urinary Hsp72 levels normalized by proteinuria. The sham group is represented by white bars; the I/R group, by black bars; and the IR + PFN group, by gray bars. Six rats per group were studied. Data are shown as the means ± SD. * = p < 0.05 vs. sham and I/R + PFN groups, + = p < 0.05 vs. I/R, & = p < 0.05 vs. sham group. The significance of the differences between groups was assessed by ANOVA using the Bonferroni correction for multiple comparisons