Fig. 6

Angptl3 knockout attenuates the ADR-induced primary podocyte loss including detachment and apoptosis, in vitro. Primary podocytes of Angptl3+/+ and Angptl3−/− mice treated with or without ADR attached to the bottom of culture dishes (100 ×, scale bars = 30 μm, n = 6), and the rates of cell attachment in the four groups were calculated (a). Podocyte apoptosis was analyzed by flow cytometry analysis (b) and a TUNEL assay (200 ×, scale bars = 15 μm, n = 6) (c). Data are shown as the mean ± SEM; n = 6 per group; **P < 0.01 and ***P < 0.001; ADR (−): podocytes with PBS treatment; ADR (+): podocytes with ADR treatment. ADR (+): podocytes with ADR treatment