Fig. 5

Effect of administration of adnisodamine/atropine on caspase-3 in rats subjected to glycerol-induced AKI. a Caspase-3 immunostaining in cross sections of rat kidney at 24 h in untreated, glycerol-treated, and adnisodamine/atropine intervention after glycerol treatment groups over the time course. Caspase-3 positive staining was observed on the proximal tubular epithelial cells and damaged tubules. U, untreated; G, glycerol treatment alone group; GAP, glycerol atropine treatment group. GAD, glycerol adnisodamine treatment group. Bars = 50 μm. Data are the mean ± SEM from three separate experiments. b. The statistic evaluation of immunostaining of caspase-3 (*P < 0.05 versus G24 group). c Total kidney tissue extracts were analyzed for caspase-3 protein levels by western blot. Data are expressed as mean ± S.E. (n = 3), statistically significant from respective 0 h controls. *, statistically significant from rats receiving glycerol treatment alone at the corresponding time point (p < 0.05)