Fig. 8
From: Protective effect of anisodamine in rats with glycerol-induced acute kidney injury
Effect of administration of adnisodamine/atropine on in rats IL-6 subjected to glycerol-induced AKI. a CD45 immunostaining in cross sections of rat kidney at 24 h in untreated, glycerol-treated, and adnisodamine/atropine intervention after glycerol treatment groups over the time course. CD45 positive staining was observed on the proximal tubular epithelial cells and damaged tubules (arrows). U, untreated; G, glycerol treatment alone group; GAP, glycerol atropine treatment group. GAD, glycerol adnisodamine treatment group. Bars = 50 μm. Data are the mean ± SEM from three separate experiments (b) The statistic evaluation of immunostaining of CD45 (*P < 0.05 versus G24 group) (c) Total kidney tissue extracts were analyzed for IL-6 protein levels by western blot. d Total kidney tissue extracts were assayed for IL-6 activity by ELISA (*P < 0.05 versus G24 group) .Data are expressed as mean ± S.E. (n = 3)