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Fig. 6 | BMC Nephrology

Fig. 6

From: Urinary nephrospheres indicate recovery from acute kidney injury in renal allograft recipients – a pilot study

Fig. 6

In situ confocal immunofluorescence and phase contrast microscopy of tissue culture grown nephrospheres. In bulk-grown nephrospheres after 20 days in tissue culture exhibited various intensity of AQP1 expression (green) and variability in cell size. AQP1+ cells covered the plate surface and were juxtaposed to AQP1- cells (a, b) and formed cell-to-cell contact. Infrequently, AQP1+ cells showed long dendritiform, podocytic extrusions (c). Proliferative foci between spreading cells were visualized by phase contrast microscopy (d) with mitotic cells (→ telophase) (e) and cells with podocytic extrusions (f)

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