Fig. 2

Confirmation of NF-κB binding ability in the rs9395890 by using ChIP assay. The representative data of enrichment of NF-κB of three genotypes, GG (n = 3), GT (n = 3), and TT (n = 3), in the rs9395890 by using chromatin immunoprecipitation assay (ChIP). Real-time qPCR was performed to measure the amount of with or without NF-κB enriched fragments. The ChIP-assay reveals that around 1.49 times enrichment of NF-κB of the variant type TT when compared to that of the wild type GG in the rs9395890. The SNP at NF-KB transcription binding site rs9395890 have high binding ability in TT type than GG type. The results are shown in % input (ChIP/input). The mean ± SEM is given for each construct from three experiments (P = 0.027; TT = 3.20 ± 0.16, GT = 2.81 ± 0.20, GG = 1.71 ± 0.18) (Table 5)