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Fig. 2 | BMC Nephrology

Fig. 2

From: Uric acid regulates NLRP3/IL-1β signaling pathway and further induces vascular endothelial cells injury in early CKD through ROS activation and K+ efflux

Fig. 2

UA induced the expression of NLRP3 complex and activation of NLRP3 inflammasome in HUVECs. The cells were treated by UA (5, 10, 20 mg/dL, respectively) for 24 h or UA (6, 12, 24 h, respectively) at 20 mg/dL. a Measurement of the expression of ASC and NLRP3 in cells and the expression of caspase 1 in cells supernatant by western blotting; b Measurement of the mRNA expression of caspase 1 and NLRP3 in cells by qPCR; c The protein expression of ASC, NLRP3, and pro-caspase 1 in cells lysates extracted by A/G immunomagnetic beaded with NLRP3 antibody was measured by western blotting; d The protein expression of ASC, NLRP3, and pro-caspase 1 in cells lysates extracted by A/G immunomagnetic beaded with ASC antibody was measured by western blotting. *P < 0.05; **p < 0.01; ***p < 0.001

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BMC Nephrology

ISSN: 1471-2369

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