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Fig. 3 | BMC Nephrology

Fig. 3

From: Uric acid regulates NLRP3/IL-1β signaling pathway and further induces vascular endothelial cells injury in early CKD through ROS activation and K+ efflux

Fig. 3

NLRP3 siRNA and inhibitor of caspase 1 affected the component of NLRP3 inflammasome and the expression of downstream cytokines. a After transfection of HUVECs with siRNA NLRP3, and subsequent incubation with UA (20 mg/dL) for 24 h, the expression of NLRP3, IL-1β, caspase 1, and ICAM-1 in cells or culture supernatant was measured by western blotting; b After 1 h pretreatment of HUVECs with caspase 1 inhibitor (10 μM Ac-YVAD-CHO), and subsequent incubation with UA (20 mg/dL) for 24 h, the expression of NLRP3, IL-1β, caspase 1, and ICAM-1 in cells or culture supernatant was measured by western blotting. *P < 0.05; **p < 0.01; ***p < 0.001

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