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Table 1 qPCR primer sequences used in this study (h: human, r: rat)

From: Uric acid regulates NLRP3/IL-1β signaling pathway and further induces vascular endothelial cells injury in early CKD through ROS activation and K+ efflux

Gene Forward primer Reverse primer
hIL-1β 5’-CTGAGCTCGCCAGTGAAATG-3’ 5’-TGTCCATGGCCACAACAACT-3’
rIL-1β 5’-CAGCAGCATCTCGACAAGAG-3’ 5’-CATCATCCCACGAGTCACAG-3’
hNLRP3 5’-AAGGCCGACACCTTGATATG-3’ 5’-CCGAATGTTACAGCCAGGAT-3’
rNLRP3 5’-GTAGGTGTGGAAGCAGGACT-3’ 5’-CTTGCTGACTGAGGACCTGA-3’
hcaspase 1 5’-CTCAGGCTCAGAAGGGAATG-3’ 5’-CGCTGTACCCCAGATTTTGT-3’
rcaspase 1 5’-CCGTGGAGAGAAACAAGGAG-3’ 5’-GGACAGGATGTCTCCAGGAC-3’
rASC 5’-TGGCTACTGCAACCAGTGTC-3’ 5’-GGCTGGAGCAAAGCTAAAGA-3’
hGAPDH 5’-CCAGGTGGTCTCCTCTGA-3’ 5’-GCTGTAGCCAAATCGTTGT-3’
rGAPDH 5’-GCAAGTTCAACGGCACAG-3’ 5’-GCCAGTAGACTCCACGACAT-3’