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Fig. 2 | BMC Nephrology

Fig. 2

From: TRPP2 dysfunction decreases ATP-evoked calcium, induces cell aggregation and stimulates proliferation in T lymphocytes

Fig. 2

Analysis of ATP- and PAF-evoked calcium in TRPP2 silenced Jurkat cells and ADPKD2 subjects currying the R872X mutation. a The expression of TRPP2 was evaluated by Western blotting in wild type Jurkat cells and transfected with TRPP2-siRNA plasmid or with scramble sequences. The calcium levels were measured after ATP (b) or PAF (c) stimulation in control (WT) and in PKD2 silenced (TRPP2-siRNA) Jurkat cells by Fura 2-AM method. The application of 100 μM ATP causes a significant reduction of cytosolic calcium release in TRPP2 silenced cells compared with control cells (80.1 ± 18.4 for TRPP2-siRNA and 154.9 ± 32.7 for WT: ***p < 0.001). No significant differences in calcium release between control and TRPP2 silenced Jurkat cells after 2 μM PAF stimulation were observed. For both ATP- and PAF-evoked calcium were performed 13 and 9 measurements in WT and TRPP2siRNA Jurkat cells, respectively. d The intracellular calcium release after ATP stimulation is lower in T lymphocytes of ADPKD2 subjects currying the R872X mutation than in CTRL (67.5 ± 12.58 for PKD2-R872X and 244.8 ± 85.5 for CTRL: ***p < 0.001). The maximal calcium concentration after ATP or PAF stimulation was calculated as Δ (delta) obtained from the maximal value minus the basal one. Data are expressed as mean ± standard deviation calculated from at least two different experiments in duplicate, while for PKD2-R872X T lymphocytes values represent the mean ± standard deviation of two experiments. PKD2-R872X = ADPKD2 subjects currying R872X mutation (n = 4). CTRL = healthy controls (n = 25). Data of ATP- and PAF-evoked calcium detected in WT and PKD2 silenced Jurkat cells are inserted in Additional file 2: Table S2

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